In September 2010, ranavirus spp. infection was responsible for mass die-offs amongst species of green frogs (Pelophylax spp.) and smooth newts in the Dwingelderveld National Park. This was the first identified case of ranavirus in the Netherlands to be reported in the literature. Ranaviruses are highly infectious and readily transmissible by human activities and, therefore, represent a significant threat to species of amphibians, reptiles and fish.